ID   NKD1_HUMAN              Reviewed;         470 AA.
AC   Q969G9; B2RC39; Q8WZ08;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   05-OCT-2010, entry version 70.
DE   RecName: Full=Protein naked cuticle homolog 1;
DE            Short=Naked-1;
DE            Short=hNkd;
DE            Short=hNkd1;
GN   Name=NKD1; Synonyms=NKD; ORFNames=PP7246;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=21255530; PubMed=11356022; DOI=10.1006/dbio.2001.0238;
RA   Wharton K.A. Jr., Zimmermann G., Rousset R., Scott M.P.;
RT   "Vertebrate proteins related to Drosophila Naked Cuticle bind
RT   Dishevelled and antagonize Wnt signaling.";
RL   Dev. Biol. 234:93-106(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP   STAGE.
RC   TISSUE=Fetus;
RX   MEDLINE=21490203; PubMed=11604995;
RA   Katoh M.;
RT   "Molecular cloning, gene structure, and expression analyses of NKD1
RT   and NKD2.";
RL   Int. J. Oncol. 19:963-969(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INDUCTION.
RX   MEDLINE=21625096; PubMed=11752446; DOI=10.1073/pnas.261574498;
RA   Yan D., Wiesmann M., Rohan M., Chan V., Jefferson A.B., Guo L.,
RA   Sakamoto D., Caothien R.H., Fuller J.H., Reinhard C., Garcia P.D.,
RA   Randazzo F.M., Escobedo J., Fantl W.J., Williams L.T.;
RT   "Elevated expression of axin2 and hnkd mRNA provides evidence that
RT   Wnt/beta-catenin signaling is activated in human colon tumors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:14973-14978(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA   Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H.,
RA   Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y.,
RA   Shu H., Chen X., Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S.,
RA   Gu J.;
RT   "Large-scale cDNA transfection screening for genes related to cancer
RT   development and progression.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Thalamus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   FUNCTION, AND INTERACTION WITH PPP2R3A.
RX   PubMed=15687260; DOI=10.1101/gad.328905;
RA   Creyghton M.P., Roeel G., Eichhorn P.J.A., Hijmans E.M., Maurer I.,
RA   Destree O., Bernards R.;
RT   "PR72, a novel regulator of Wnt signaling required for Naked cuticle
RT   function.";
RL   Genes Dev. 19:376-386(2005).
RN   [9]
RP   FUNCTION, AND INTERACTION WITH DVL1; DVL2; DVL3 AND PPP2R3A.
RX   PubMed=16567647; DOI=10.1073/pnas.0507237103;
RA   Creyghton M.P., Roeel G., Eichhorn P.J.A., Vredeveld L.C., Destree O.,
RA   Bernards R.;
RT   "PR130 is a modulator of the Wnt-signaling cascade that counters
RT   repression of the antagonist Naked cuticle.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:5397-5402(2006).
CC   -!- FUNCTION: Cell autonomous antagonist of the canonical Wnt
CC       signaling pathway. May activate a second Wnt signaling pathway
CC       that controls planar cell polarity.
CC   -!- SUBUNIT: Interacts with DVL1, DVL2, DVL3 and PPP2R3A.
CC   -!- INTERACTION:
CC       Q06190:PPP2R3A; NbExp=3; IntAct=EBI-1538217, EBI-949190;
CC   -!- SUBCELLULAR LOCATION: Cell membrane (By similarity). Cytoplasm (By
CC       similarity).
CC   -!- TISSUE SPECIFICITY: Expressed in colon, heart, kidney, leukocyte,
CC       liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle,
CC       small intestine and spleen.
CC   -!- DEVELOPMENTAL STAGE: Expressed in fetal brain, kidney, liver and
CC       lung.
CC   -!- INDUCTION: Expression is induced by activation of the Wnt
CC       signaling pathway.
CC   -!- SIMILARITY: Belongs to the NKD family.
CC   -!- SIMILARITY: Contains 1 EF-hand domain.
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DR   EMBL; AF358135; AAK57484.1; -; mRNA.
DR   EMBL; AB062886; BAB70500.1; -; mRNA.
DR   EMBL; AY061883; AAL32374.1; -; mRNA.
DR   EMBL; AF289584; AAL55768.1; -; mRNA.
DR   EMBL; AK314930; BAG37436.1; -; mRNA.
DR   EMBL; CH471092; EAW82761.1; -; Genomic_DNA.
DR   EMBL; BC051288; AAH51288.1; -; mRNA.
DR   IPI; IPI00056339; -.
DR   RefSeq; NP_149110.1; -.
DR   UniGene; Hs.592059; -.
DR   ProteinModelPortal; Q969G9; -.
DR   IntAct; Q969G9; 12.
DR   STRING; Q969G9; -.
DR   PRIDE; Q969G9; -.
DR   Ensembl; ENST00000268459; ENSP00000268459; ENSG00000140807.
DR   GeneID; 85407; -.
DR   KEGG; hsa:85407; -.
DR   UCSC; uc002egg.1; human.
DR   CTD; 85407; -.
DR   GeneCards; GC16P050629; -.
DR   H-InvDB; HIX0013025; -.
DR   H-InvDB; HIX0027004; -.
DR   HGNC; HGNC:17045; NKD1.
DR   MIM; 607851; gene.
DR   PharmGKB; PA31637; -.
DR   eggNOG; prNOG17172; -.
DR   HOGENOM; HBG444925; -.
DR   HOVERGEN; HBG099718; -.
DR   InParanoid; Q969G9; -.
DR   OMA; YHHFYQT; -.
DR   OrthoDB; EOG9HQH4K; -.
DR   PhylomeDB; Q969G9; -.
DR   Pathway_Interaction_DB; wnt_canonical_pathway; Canonical Wnt signaling pathway.
DR   NextBio; 75970; -.
DR   ArrayExpress; Q969G9; -.
DR   Bgee; Q969G9; -.
DR   CleanEx; HS_NKD1; -.
DR   Genevestigator; Q969G9; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR011992; EF-hand-like_dom.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR018249; EF_HAND_2.
DR   Gene3D; G3DSA:1.10.238.10; EF-Hand_type; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 1.
PE   1: Evidence at protein level;
KW   Calcium; Cell membrane; Complete proteome; Cytoplasm; Lipoprotein;
KW   Membrane; Myristate; Wnt signaling pathway.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    470       Protein naked cuticle homolog 1.
FT                                /FTId=PRO_0000301990.
FT   DOMAIN      131    166       EF-hand.
FT   CA_BIND     144    155       Potential.
FT   REGION      125    190       Interaction with DVL1, DVL2 and DVL3 (By
FT                                similarity).
FT   COMPBIAS    452    466       His-rich.
FT   LIPID         2      2       N-myristoyl glycine (By similarity).
FT   CONFLICT      9     10       AA -> VD (in Ref. 4; AAL55768).
SQ   SEQUENCE   470 AA;  52285 MW;  E50E528042662CDE CRC64;
     MGKLHSKPAA VCKRRESPEG DSFAVSAAWA RKGIEEWIGR QRCPGGVSGP RQLRLAGTIG
     RSTRELVGDV LRDTLSEEEE DDFRLEVALP PEKTDGLGSG DEKKMERVSE PCPGSKKQLK
     FEELQCDVSM EEDSRQEWTF TLYDFDNNGK VTREDITSLL HTIYEVVDSS VNHSPTSSKM
     LRVKLTVAPD GSQSKRSVLV NQADLQSARP RAETKPTEDL RSWEKKQRAP LRFQGDSRLE
     QSGCYHHCVD ENIERRNHYL DLAGIENYTS QFGPGSPSVA QKSELPPRTS NPTRSRSHEP
     EAIHIPHRKP QGVDPASFHF LDTPIAKVSE LQQRLRGTQD GSKHFVRSPK AQGKSVGVGH
     VARGARNKPP LGPAIPAVSP SAHLAASPAL LPSLAPLGHK KHKHRAKESQ QGCRGLQAPL
     ASGGPVLGRE HLRELPALVV YESQAGQPVQ RHEHHHHHEH HHHYHHFYQT
//