ID   DGKB_HUMAN              Reviewed;         804 AA.
AC   Q9Y6T7; A4D116; A4D117; A8MXU2; O75241; Q2M377; Q75MF9; Q75MU7;
AC   Q86UI5; Q86UM9; Q9UQ29;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2001, sequence version 2.
DT   05-OCT-2010, entry version 93.
DE   RecName: Full=Diacylglycerol kinase beta;
DE            Short=DAG kinase beta;
DE            EC=2.7.1.107;
DE   AltName: Full=90 kDa diacylglycerol kinase;
DE   AltName: Full=Diglyceride kinase beta;
DE            Short=DGK-beta;
GN   Name=DGKB; Synonyms=DAGK2, KIAA0718;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Caricasole A., Caldara F., Sala C.F.;
RT   "Novel proteins.";
RL   Patent number WO0047723, 17-AUG-2000.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22737999; PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R.,
RA   Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H.,
RA   Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A.,
RA   Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J.,
RA   Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A.,
RA   Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S.,
RA   Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M.,
RA   Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C.,
RA   Latreille P., Miller N., Johnson D., Murray J., Woessner J.P.,
RA   Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J.,
RA   Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L.,
RA   Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R.,
RA   Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K.,
RA   Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S.,
RA   Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M.,
RA   Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R.,
RA   Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D.,
RA   Waterston R.H., Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22616434; PubMed=12690205; DOI=10.1126/science.1083423;
RA   Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA   Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA   Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA   Kanematsu E., Gentles S., Christopoulos C.C., Choufani S.,
RA   Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z.,
RA   Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C.,
RA   Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J.,
RA   Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F.,
RA   Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F.,
RA   Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H.,
RA   Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G.,
RA   Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P.,
RA   Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J.,
RA   Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F.,
RA   Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B.,
RA   Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA   Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W.,
RA   Mural R.J., Adams M.D., Tsui L.-C.;
RT   "Human chromosome 7: DNA sequence and biology.";
RL   Science 300:767-772(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-804 (ISOFORM 1).
RC   TISSUE=Brain;
RX   MEDLINE=99087487; PubMed=9872452; DOI=10.1093/dnares/5.5.277;
RA   Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A.,
RA   Kotani H., Nomura N., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. XI.
RT   The complete sequences of 100 new cDNA clones from brain which code
RT   for large proteins in vitro.";
RL   DNA Res. 5:277-286(1998).
CC   -!- FUNCTION: Exhibits high phosphorylation activity for long-chain
CC       diacylglycerols (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-
CC       sn-glycerol 3-phosphate.
CC   -!- ENZYME REGULATION: Stimulated by phosphatidylserine (By
CC       similarity).
CC   -!- INTERACTION:
CC       Q15323:KRT31; NbExp=1; IntAct=EBI-1049421, EBI-948001;
CC       Q9Y5E4:PCDHB5; NbExp=1; IntAct=EBI-1049421, EBI-1045222;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9Y6T7-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9Y6T7-2; Sequence=VSP_021898, VSP_021899;
CC         Note=No experimental confirmation available;
CC   -!- SIMILARITY: Belongs to the eukaryotic diacylglycerol kinase
CC       family.
CC   -!- SIMILARITY: Contains 1 DAGKc domain.
CC   -!- SIMILARITY: Contains 2 EF-hand domains.
CC   -!- SIMILARITY: Contains 2 phorbol-ester/DAG-type zinc fingers.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAS07533.1; Type=Erroneous gene model prediction;
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DR   EMBL; AX032742; CAC09945.1; -; mRNA.
DR   EMBL; AC005039; AAS07495.1; -; Genomic_DNA.
DR   EMBL; AC005248; AAC25525.1; -; Genomic_DNA.
DR   EMBL; AC006150; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC011229; AAP21868.1; -; Genomic_DNA.
DR   EMBL; AC073258; AAS07533.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AC105459; AAP22362.1; -; Genomic_DNA.
DR   EMBL; CH236948; EAL24292.1; -; Genomic_DNA.
DR   EMBL; CH236948; EAL24293.1; -; Genomic_DNA.
DR   EMBL; CH471073; EAW93663.1; -; Genomic_DNA.
DR   EMBL; CH471073; EAW93664.1; -; Genomic_DNA.
DR   EMBL; BC105005; AAI05006.1; -; mRNA.
DR   EMBL; AB018261; BAA34438.1; -; mRNA.
DR   IPI; IPI00002590; -.
DR   IPI; IPI00893505; -.
DR   RefSeq; NP_004071.1; -.
DR   RefSeq; NP_663733.1; -.
DR   UniGene; Hs.567255; -.
DR   ProteinModelPortal; Q9Y6T7; -.
DR   SMR; Q9Y6T7; 5-157, 8-229, 157-297, 243-312, 303-363, 438-775.
DR   IntAct; Q9Y6T7; 2.
DR   STRING; Q9Y6T7; -.
DR   PhosphoSite; Q9Y6T7; -.
DR   PRIDE; Q9Y6T7; -.
DR   Ensembl; ENST00000399322; ENSP00000382260; ENSG00000136267.
DR   Ensembl; ENST00000403951; ENSP00000385780; ENSG00000136267.
DR   GeneID; 1607; -.
DR   KEGG; hsa:1607; -.
DR   UCSC; uc003ssz.1; human.
DR   UCSC; uc003sta.1; human.
DR   CTD; 1607; -.
DR   GeneCards; GC07M014153; -.
DR   HGNC; HGNC:2850; DGKB.
DR   MIM; 604070; gene.
DR   PharmGKB; PA27311; -.
DR   HOVERGEN; HBG051345; -.
DR   InParanoid; Q9Y6T7; -.
DR   OMA; SCVVIRT; -.
DR   OrthoDB; EOG9VQD83; -.
DR   BRENDA; 2.7.1.107; 247.
DR   Reactome; REACT_14797; Signaling by GPCR.
DR   Reactome; REACT_604; Hemostasis.
DR   DrugBank; DB00144; Phosphatidylserine.
DR   NextBio; 6600; -.
DR   ArrayExpress; Q9Y6T7; -.
DR   Bgee; Q9Y6T7; -.
DR   Genevestigator; Q9Y6T7; -.
DR   GermOnline; ENSG00000136267; Homo sapiens.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0004143; F:diacylglycerol kinase activity; IEA:EC.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0007205; P:activation of protein kinase C activity by ...; IEA:InterPro.
DR   InterPro; IPR000756; Diacylglycerol_kin_accessory.
DR   InterPro; IPR001206; Diacylglycerol_kinase_cat_dom.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR018249; EF_HAND_2.
DR   InterPro; IPR002048; EF_hand_Ca-bd.
DR   InterPro; IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
DR   Pfam; PF00130; C1_1; 2.
DR   Pfam; PF00609; DAGK_acc; 1.
DR   Pfam; PF00781; DAGK_cat; 1.
DR   SMART; SM00109; C1; 2.
DR   SMART; SM00045; DAGKa; 1.
DR   SMART; SM00046; DAGKc; 1.
DR   SMART; SM00054; EFh; 2.
DR   PROSITE; PS50146; DAGK; 1.
DR   PROSITE; PS00018; EF_HAND_1; 2.
DR   PROSITE; PS50222; EF_HAND_2; 2.
DR   PROSITE; PS00479; ZF_DAG_PE_1; 2.
DR   PROSITE; PS50081; ZF_DAG_PE_2; 2.
PE   1: Evidence at protein level;
KW   Alternative splicing; ATP-binding; Calcium; Complete proteome;
KW   Cytoplasm; Kinase; Metal-binding; Nucleotide-binding; Polymorphism;
KW   Repeat; Transferase; Zinc; Zinc-finger.
FT   CHAIN         1    804       Diacylglycerol kinase beta.
FT                                /FTId=PRO_0000218457.
FT   DOMAIN      149    184       EF-hand 1.
FT   DOMAIN      194    229       EF-hand 2.
FT   DOMAIN      434    568       DAGKc.
FT   CA_BIND     162    173       1 (Potential).
FT   CA_BIND     207    218       2 (Potential).
FT   ZN_FING     244    294       Phorbol-ester/DAG-type 1.
FT   ZN_FING     309    358       Phorbol-ester/DAG-type 2.
FT   VAR_SEQ     770    773       IKIT -> VSTE (in isoform 2).
FT                                /FTId=VSP_021898.
FT   VAR_SEQ     774    804       Missing (in isoform 2).
FT                                /FTId=VSP_021899.
FT   VARIANT     789    789       G -> S (in dbSNP:rs34616903).
FT                                /FTId=VAR_048858.
SQ   SEQUENCE   804 AA;  90595 MW;  CBCABD2339BFE176 CRC64;
     MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK QDILNQTIDF
     EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPMVKS KPALLSGGLR MNKGAITPPR
     TTSPANTCSP EVIHLKDIVC YLSLLERGRP EDKLEFMFRL YDTDGNGFLD SSELENIISQ
     MMHVAEYLEW DVTELNPILH EMMEEIDYDH DGTVSLEEWI QGGMTTIPLL VLLGLENNVK
     DDGQHVWRLK HFNKPAYCNL CLNMLIGVGK QGLCCSFCKY TVHERCVARA PPSCIKTYVK
     SKRNTDVMHH YWVEGNCPTK CDKCHKTVKC YQGLTGLHCV WCQITLHNKC ASHLKPECDC
     GPLKDHILPP TTICPVVLQT LPTSGVSVPE ERQSTVKKEK SGSQQPNKVI DKNKMQRANS
     VTVDGQGLQV TPVPGTHPLL VFVNPKSGGK QGERIYRKFQ YLLNPRQVYS LSGNGPMPGL
     NFFRDVPDFR VLACGGDGTV GWVLDCIEKA NVGKHPPVAI LPLGTGNDLA RCLRWGGGYE
     GENLMKILKD IENSTEIMLD RWKFEVIPND KDEKGDPVPY SIINNYFSIG VDASIAHRFH
     IMREKHPEKF NSRMKNKFWY FEFGTSETFS ATCKKLHESV EIECDGVQID LINISLEGIA
     ILNIPSMHGG SNLWGESKKR RSHRRIEKKG SDKRTTVTDA KELKFASQDL SDQLLEVVGL
     EGAMEMGQIY TGLKSAGRRL AQCSCVVIRT SKSLPMQIDG EPWMQTPCTI KITHKNQAPM
     LMGPPPKTGL FCSLVKRTRN RSKE
//